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KMID : 0357319850200020213
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Journal of the Korean Society for Microbiology
1985 Volume.20 No. 2 p.213 ~ p.220
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Active E-rosette Forming Responses of Peripheral Blood Lymphocytes from Patients with Pulmonary Tuberculosis after in vitro PPD Stimulation
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Abstract
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The present study was undertaken to assess in vitro cell-mediated immunity (CMI) of patients with tuberculosis to purified protein derivative (PPD). CMI was assessed by active E-rosette forming cell(ARFC) response and leukocyte migration inhibition test. Patients with tuberculosis were divided into two polar group: clinically localized reactive (RR) group and clinically diffuse unreactive(UU) group, the former characterized by good delayed-type hypersensitivity (DTH) but poor anti-PPD antibodies, and the latter by poor DTH but ample -anti-PPD antibodies. These two groups of patients were subjected to the present experiment.
The peripheral blood lymphocyte (PBL) purified from RR-and UU-groups or PPD skin test positive or negative healthy donors were incubated in the presence or absence of PPD (100ng/ml). The A- RF C response was evaluated at 1, 2, 4 and 6 hrs after incubation. The PBL from PPD skin test positive healthy donors or RR group when cultured with PPD produced a significant increase of the ability of the lymphocyte to form A-RFC and leukocyte inhibitory factor (LIF) with inhibited the migration of chicken buffy coat leukocytes as compared to lymphocytes cultured without antigen. However, PBL from PPD skin test negative healthy donors and UU group of patients even cultured with PPD did not increase in the ability to form A-RFC and LIF when compared to PBL cultured without antigen. This result may confirm and extend that this assay may be a new in vitro method for investigating mechanism of CMI and rapid diagnostic test for sensitization to Mycobacterium tuberculosis.
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KEYWORD
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